You can use Glade, you can probably use any version of Gtk with Python3, Python doesn't care which version you use (as far as I know).
For an example of how Python and Gtk work together, paying special attention to the attached files, you might want to look at this post:viewtopic.php?f=12&t=11926
For a newer version of the example project, again paying special attention to the attached files, try this:http://www.gtkforums.com/viewtopic.php? ... a90ab89f07
In the future, try not to be rude.